Like most Rotary clubs, RC of Ybor City, organizes its activities around Rotary's "Four Avenues of Service," which refer to the four elements of the Object of Rotary:

• "Club Service" involves all of the activities necessary for Rotarians to perform in order to make their club function successfully.
  
• "Vocational Service" is a description of the opportunity each Rotarian has to represent the dignity and utility of one's vocation to the other members of the club. Additionally, Vocational Service presents an opportunity for Rotarians to share Rotary ideals such as the four-way test with non-Rotarians in the workplace.
  
• "Community Service" pertains to those activities which Rotarians undertake to improve the quality of life in their community. It frequently involves assistance to youth, the aged, handicapped and others who look to Rotary as a source of hope for a better life.
  
• The Fourth Avenue, "International Service," describes the many programs and activities which Rotarians undertake to advance international understanding, goodwill and peace. International Service projects are designed to meet humanitarian needs of people in many lands.